LOCUS       sequence-127931-         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the CaMV 35S terminator as a level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pT35S (GB0036)
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(385..403)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(386..403)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(434..456)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    445..461
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(469..475,476..493,494..499))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    514..535
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctccggccgc catggcggcc gcgggaattc gattgggcga tgagtggtct cggcttcggc
       61 catgctagag tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt
      121 ctccagaata atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg
      181 ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta
      241 tcaataaaat ttctaattcc taaaaccaaa atccagtgac ctcgctcgag accactcatc
      301 gcccaatcac tagtganttc gcggccgcct gcaggtcgac catatgggag agctcccaac
      361 gcgttggatg cntagcttga gnattctata gtgtcaccta aatagcttgg cgtaatcatg
      421 gncanagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      481 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      541 gttgcgctca ctgcccgctt tccagtcngg aaacctgtcg tgccagctgc attaatgaat
      601 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
      661 tgactc
//
