LOCUS       sequence-127932-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-4 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2228
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      70..259
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    307..340
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        395..738
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 aagaaattcg cttatttaga agtgtcaaca acgtatctac caacggaatg cgtgcgatcg
       61 cgtgcattca tccgctctaa ccgaaaagga aggagttaga caacctgaag tctaggtccc
      121 tatttatttt tttatagtta tgttagtatt aagaacgtta tttatatttc aaatttttct
      181 tttttttctg tacagacgcg tgtacgcatg taacattata ctgaaaacct tgcttgagaa
      241 ggttttggga cgctcgaaga tcgcgtcagc tgaagcttcg tacgctgcag gtcgacaacc
      301 cttaatataa cttcgtataa tgtatgctat acgaagttat taggtctaga gatctgttta
      361 gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct
      421 tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc
      481 catacatccc catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag
      541 caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc
      601 gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact
      661 gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac
      721 atccgaacat aaacaaccat gggtaggagg gcttttgtag aaagaaatac gaacgaaacg
      781 aaaatcagcg ttgccatcgc tttggacaaa gctcccttac ctgaagagtc gaattttatt
      841 gatgaactta taacttccaa gcatgcaaac caaaagggag aacaagtaat ccaagtagac
      901 acgggaattg gat
//
