LOCUS       sequence-127935-         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-J2
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(37..68)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 gagtggcagg gcggggcgta aacgccatgg gcatgtagtc aaaagcctcc ggtcggaggc
       61 ttttgacttg gctgaggagt gccgttaatt aagtttaaac gtaccttgcc atcttgccag
      121 catcaacttc cagccaggtt ggcttttcac gctgctcagc cagctccaga gcggctttca
      181 cgcgagactg cttcttcgct ttctcacgaa tgcttacaac gtcattcgga ctaacctgat
      241 aagaagcgat gttaacaaca cgaccgttta ccataattgc tttatggcta accagctgac
      301 gtgcttctgc acgagtggca ccgaagccca tacggtatac aacgttgtcc agacgacctt
      361 ccagcagagc caacaggttt tcaccggtgt tgcctttcag acgtgctgct tctttgtagt
      421 agttacggaa ctgacgctcc agcacaccat agatacggcg aactttttgc ttttcacgca
      481 actgcacacc atagtcagac agacgcggtt tacgcgcacc gtgctggcca ggagcttgtt
      541 caattttaca cttggtatcg atcgcgcgaa cgccagactt aaggaataag tcggtgccct
      601 cacgacggct cagcttgagc ttaggaccca aatatcttgc catagcggtc atttatgtca
      661 gacttgagtt gtacgcgtcc aaa
//
