LOCUS       sequence-127944-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked amdSYM marker, integration into S. cerevisiae XI-5 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2399
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     67..84
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     68..84
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    554..587
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gcatcaaact aagcagaagg cccctgacgg atggcctttt tgcgtttcta caaactcttt
       61 ctgtgttgta aaacgacggc cagtcttaag ctcgggcccc ctgggcggtt ctgataacga
      121 gtaatcgtta atccgcaaat aacgtaaaaa cccgcttcgg cgggtttttt tatgggggga
      181 gtttagggaa agagcatttg tcagaatatt taagggcgcc tgtcactttg cttgatatat
      241 gagaattatt taaccttata aatgagaaaa aagcaacgca ctttaaataa gatacgttgc
      301 tttttcgatt gatgaacacc tataattaaa ctattcatct attatttatg attttttgta
      361 tatacaatat ttctagtttg ttaaagagaa ttaagaaaat aaatctcgaa aataataaag
      421 ggaaaatcag tttttgatat caaaattata catgtcaacg ataatacaaa atataataca
      481 aactataaga tgttatcagt atttattatg catttagaat aaattttgtg tcgcccttat
      541 tcgactcact atagaagttc ctattctcta gaaagtatag gaacttcact tcattttatt
      601 taaatttgcg gccgcgctga ggtcttaatg atcatagatc cggcacttag agaaagacca
      661 tagttgacca acttagattg gctattgcgc ttgctatcga aggcaaccga taacaacggc
      721 acacacaggg ccccaagtat gcacagcacc gacgatataa gagaagacaa caaccacctg
      781 ggaatttttt ccattttctt gcgtttcttc actgtgatgc ttgtgagaat attaggatat
      841 accaacgtgc aactctgaaa tgtcaaaacg gtcgtgtata aataaatgat ataccagtag
      901 gtgaaatcat ctcatttgtc tttctaaaaa agtccattac ccttaaggtt ggtc
//
