LOCUS       sequence-127945-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked amdSYM marker, integration into S. cerevisiae XI-5 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2399
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    167..200
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggac tccccatgcg
       61 agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      121 tcgcccgggc taattatggg gtgtcgccct tattcgactc tatagtgaag ttcctattct
      181 ctagaaagta taggaacttc tgaagtgggg atttaaatgc ggccgcgctg agggtttaat
      241 gcggagaagt cgttgatagc atttccgaag gcttttccac attgactgca gtatctaaaa
      301 gagctccagc agcggtctta gtagttcctg tgcttgccgc tttgcagtat acagggacat
      361 tgggaactac ggaggaaaaa tcggaatgct ctgagctctt ctcaaaacca gacgaactcg
      421 tcttatttct cagtaaatta cccaacatat aaactctccc tcttcacctt ctctacaagg
      481 ccacacacaa gacagtttta acaggcaaat ggtactggag gctctcacca atggggttta
      541 agacgctcca aacacacgct ctcctttttt tctttatata tcggtagcaa cgaaagctag
      601 tcgcagaacg ttacacacta aagcagatca atcaatcaat cctgagaacc tgcttcgttc
      661 aaaccatcca ctagaagaaa cacgcacgtg tacccaaaaa tagagacaaa caaacagaca
      721 aaaataacaa tgattcctac cgactaaagt aaaacaacac tcacgtaata aaaaatccta
      781 gatacaactc acttgtattc ctctcttctt tgaccatcac taaagcttct ctcttactat
      841 attcctcaaa taaaaaaaag ttgcaatgat tacttaccaa tgtgccataa actccgtgca
      901 ccaattaagt cc
//
