LOCUS       sequence-127946-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked natMX marker, integration into S. cerevisiae XII-1 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2197
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      94..283
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    331..364
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        419..762
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 ttaataataa aaatcataaa tcataagaaa ttcgcttatt tagaagtgtc aacaacgtat
       61 ctaccaacgg aatgcgtgcg atcgcgtgca ttcatccgct ctaaccgaaa aggaaggagt
      121 tagacaacct gaagtctagg tccctattta tttttttata gttatgttag tattaagaac
      181 gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat
      241 tatactgaaa accttgcttg agaaggtttt gggacgctcg aagatcgcgt cagctgaagc
      301 ttcgtacgct gcaggtcgac aacccttaat ataacttcgt ataatgtatg ctatacgaag
      361 ttattaggtc tagagatctg tttagcttgc ctcgtccccg ccgggtcacc cggccagcga
      421 catggaggcc cagaataccc tccttgacag tcttgacgtg cgcagctcag gggcatgatg
      481 tgactgtcgc ccgtacattt agcccataca tccccatgta taatcatttg catccataca
      541 ttttgatggc cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga cctgcgagca
      601 gggaaacgct cccctcacag acgcgttgaa ttgtccccac gccgcgcccc tgtagagaaa
      661 tataaaaggt taggatttgc cactgaggtt cttctttcat atacttcctt ttaaaatctt
      721 gctaggatac agttctcaca tcacatccga acataaacaa ccatgggtac cactcttgac
      781 gacacggctt accggtaccg caccagtgtc ccgggggacg ccgaggccat cgaggcactg
      841 gatgggtcct tcaccaccga caccgttttc cgcgtcaccg ccaccgggga cggcttcacc
      901 ctgcgggagg tgccggtgga cccgcccctg accaaggtgt tccccgacga cgaatcggac
      961 gacgaatcgg acgacgggga ggacggcgac
//
