LOCUS       sequence-127947-        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked natMX marker, integration into S. cerevisiae XII-1 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2197
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    182..215
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      complement(808..973)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     894..918
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
       61 gggactcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
      121 gaaagactgg gcctttcgcc cgggctaatt atggggtgtc gcccttattc gactctatag
      181 tgaagttcct attctctaga aagtatagga acttctgaag tggggattta aatgcggccg
      241 cgctgagggt ttaatgttga gctctgtcct tcatggactt tttggaccac ttcttcttgg
      301 acttcttacc accagcaaga gcggcagcgg ctttggcagc tttagacaat tgttgctttg
      361 gaggcatgtt atattatgtt ctgagacgta agaaagggtg aaaattgatg ttagtgtcaa
      421 aaattatatt acaaaatacg cagagatatt ctagttcctt tgatgaatga atctttcaga
      481 aaaaaaagtc aaagcaaaag caaaatggcc tgcagactaa actgtatggt ggtcttggaa
      541 tgataaagat ctgtttaata gatttagtag atacaatagc acatctcatt acccagttat
      601 gattgacgtc attctgagtt acaatgatct tagcgacctg tgctcattat ttgctccact
      661 aattctaatt ttcctcgcct ttcatatttc gtatctttat tctatatcct aaaatttttt
      721 tggcaaatcc cagatttggc tttgattttg gcatcggttc ggttctttca ttaagtcctc
      781 agcgagctcg catggaatgc gtgcgatgag cgacctcatg ctatacctga gaaagcaacc
      841 tgacctacag gaaagagtta ctcaagaata agaattttcg ttttaaaacc taagagtcac
      901 tttaaaattt gtatacactt atttttttta taacttattt aataataaaa atcataaatc
      961 ataagaaatt cgcttattta gaagtgtcaa caacgtatct ac
//
