LOCUS       sequence-127950-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked natMX marker, integration into S. cerevisiae XII-1 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2197
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     71..88
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     72..88
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    558..591
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ccaggcatca aactaagcag aaggcccctg acggatggcc tttttgcgtt tctacaaact
       61 ctttctgtgt tgtaaaacga cggccagtct taagctcggg ccccctgggc ggttctgata
      121 acgagtaatc gttaatccgc aaataacgta aaaacccgct tcggcgggtt tttttatggg
      181 gggagtttag ggaaagagca tttgtcagaa tatttaaggg cgcctgtcac tttgcttgat
      241 atatgagaat tatttaacct tataaatgag aaaaaagcaa cgcactttaa ataagatacg
      301 ttgctttttc gattgatgaa cacctataat taaactattc atctattatt tatgattttt
      361 tgtatataca atatttctag tttgttaaag agaattaaga aaataaatct cgaaaataat
      421 aaagggaaaa tcagtttttg atatcaaaat tatacatgtc aacgataata caaaatataa
      481 tacaaactat aagatgttat cagtatttat tatgcattta gaataaattt tgtgtcgccc
      541 ttattcgact cactatagaa gttcctattc tctagaaagt ataggaactt cacttcattt
      601 tatttaaatt tgcggccgcg ctgaggtctt aatcaatcct cgcatttcag cttccattat
      661 atttgatgac tgtttctcaa tctttatgtt atcttcttac accgtatgtg atgatatact
      721 aataacacgg atacttaata aacagatgat gttacagttt catccaatac gaacgattat
      781 caaattattg caacatttcc cagtgaaatc ttcaccaaat gctcatacag cgtgacaata
      841 atgagcattt tgaattttaa ggaaattaat ttgtcctcta tcttttgcat ccgtgcctcc
      901 tggataataa cttcaatcct tgcaccacca ttgaacttgt aacagagctt tttttttaac
      961 ttcttgtcct ggtactgcat aatgaatagc gccagaagtt
//
