LOCUS       sequence-127953-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked dsdA marker, integration into S. cerevisiae XII-1 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2400
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      74..263
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    311..344
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        399..742
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 tcataagaaa ttcgcttatt tagaagtgtc aacaacgtat ctaccaacgg aatgcgtgcg
       61 atcgcgtgca ttcatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg
      121 tccctattta tttttttata gttatgttag tattaagaac gttatttata tttcaaattt
      181 ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg
      241 agaaggtttt gggacgctcg aagatcgcgt cagctgaagc ttcgtacgct gcaggtcgac
      301 aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc tagagatctg
      361 tttagcttgc cttgtccccg ccgggtcacc cggccagcga catggaggcc cagaataccc
      421 tccttgacag tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc ccgtacattt
      481 agcccataca tccccatgta taatcatttg catccataca ttttgatggc cgcacggcgc
      541 gaagcaaaaa ttacggctcc tcgctgcaga cctgcgagca gggaaacgct cccctcacag
      601 acgcgttgaa ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt taggatttgc
      661 cactgaggtt cttctttcat atacttcctt ttaaaatctt gctaggatac agttctcaca
      721 tcacatccga acataaacaa ccatggaaaa cgctaaaatg aactcgctca tcgcccagta
      781 tccgttggta aaggatctgg ttgctcttaa agaaaccacc tggtttaatc ctggcacgac
      841 ctcattggct gaaggtttac cttatgttgg cctgaccgaa caggatgttc aggacgccca
      901 tgcgcgctta tcccgttttg caccctatct ggcaaaagca tttcctgaaa ctgctgccac
      961 tggggggatt attgaatca
//
