LOCUS       sequence-127955-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked dsdA marker, integration into S. cerevisiae XII-1 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2400
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..85
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     69..85
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    555..588
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ggcatcaaac taagcagaag gcccctgacg gatggccttt ttgcgtttct acaaactctt
       61 tctgtgttgt aaaacgacgg ccagtcttaa gctcgggccc cctgggcggt tctgataacg
      121 agtaatcgtt aatccgcaaa taacgtaaaa acccgcttcg gcgggttttt ttatgggggg
      181 agtttaggga aagagcattt gtcagaatat ttaagggcgc ctgtcacttt gcttgatata
      241 tgagaattat ttaaccttat aaatgagaaa aaagcaacgc actttaaata agatacgttg
      301 ctttttcgat tgatgaacac ctataattaa actattcatc tattatttat gattttttgt
      361 atatacaata tttctagttt gttaaagaga attaagaaaa taaatctcga aaataataaa
      421 gggaaaatca gtttttgata tcaaaattat acatgtcaac gataatacaa aatataatac
      481 aaactataag atgttatcag tatttattat gcatttagaa taaattttgt gtcgccctta
      541 ttcgactcac tatagaagtt cctattctct agaaagtata ggaacttcac ttcattttat
      601 ttaaatttgc ggccgcgctg aggtcttaat caatcctcgc atttcagctt ccattatatt
      661 tgatgactgt ttctcaatct ttatgttatc ttcttacacc gtatgtgatg atatactaat
      721 aacacggata cttaataaac agatgatgtt acagtttcat ccaatacgaa cgattatcaa
      781 attattgcaa catttcccag tgaaatcttc accaaatgct catacagcgt gacaataatg
      841 agcattttga attttaagga aattaatttg tcctctatct tttgcatccg tgcctcctgg
      901 ataataactt caatcctt
//
