LOCUS       sequence-127957-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked dsdA marker, integration into S. cerevisiae XII-1 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2400
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    152..185
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gtgaaacgcc gtagcgccga tggtagtgtg gggactcccc atgcgagagt agggaactgc
       61 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgcc cgggctaatt
      121 atggggtgtc gcccttattc gactctatag tgaagttcct attctctaga aagtatagga
      181 acttctgaag tggggattta aatgcggccg cgctgagggt ttaatgttga gctctgtcct
      241 tcatggactt tttggaccac ttcttcttgg acttcttacc accagcaaga gcggcagcgg
      301 ctttggcagc tttagacaat tgttgctttg gaggcatgtt atattatgtt ctgagacgta
      361 agaaagggtg aaaattgatg ttagtgtcaa aaattatatt acaaaatacg cagagatatt
      421 ctagttcctt tgatgaatga atctttcaga aaaaaaagtc aaagcaaaag caaaatggcc
      481 tgcagactaa actgtatggt ggtcttggaa tgataaagat ctgtttaata gatttagtag
      541 atacaatagc acatctcatt acccagttat gattgacgtc attctgagtt acaatgatct
      601 tagcgacctg tgctcattat ttgctccact aattctaatt ttcctcgcct ttcatatttc
      661 gtatctttat tctatatcct aaaatttttt tggcaaatcc cagatttggc tttgattttg
      721 gcatcggttc ggttctttca ttaagtcctc agcgagctcg catggaatgc gtgcgatgag
      781 cgacctcatg ctatacctga gaaagcaacc tgacctacag gaaagagtta ctcaagaata
      841 agaattttcg ttttaaaacc taagagtcac tttaaaattt g
//
