LOCUS       sequence-127958-        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of lyso-pHluorin in mammalian cells for optical readout 
            of lysosomal acidification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Rost BR, Schneider F, Grauel MK, Wozny C, G Bentz C, Blessing A, 
            Rosenmund T, Jentsch TJ, Schmitz D, Hegemann P, Rosenmund C
  TITLE     Optogenetic acidification of synaptic vesicles and lysosomes.
  JOURNAL   Nat Neurosci. 2015 Nov 9. doi: 10.1038/nn.4161.
  PUBMED    26551543
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 Nov 9. doi: 10.1038/nn.4161."
COMMENT     Sequence Label: pCMV-lyso-pHluorin
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    133..254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(144..163)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     198..217
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(288..314)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(958..978)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
       61 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
      121 caatttacgc cttaagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
      181 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
      241 gcaataaaca agttaacaac aacaattcgc ccttgcttaa ttaactaagc gtaatctgga
      301 acatcgtatg ggtacattac ttcatagcca cttcgaatac tcttcaccag acagcaggag
      361 aagataattc ccaagacctc cacaaaagca atgcccaggg ccgctgcagc caccagcagt
      421 atgttcttcc ttagccatat tgctatagtc tccacgcagc cctgggtatg gatagtggat
      481 tccttgaaat cattcccaca gcccacagtt atgttgatgc agcaagaatc ggggactctg
      541 tccttggcca tgccggggat gttttcccag tctgtgtagt tagaagctcc acagcagtta
      601 ttttctttct gcaatttgtc caaaatagtg gctgttttgt tgtctttaag gtaattctgc
      661 atctgctgct ggaagctttt attaaactct gacttcacct ggtctctaaa cacatagcca
      721 gcaatggcca cagccacctc cacaagcatg ataagagaca ggaagatggc aaatgtaatc
      781 atgagacagt agttctcctt gcaggcccca cagcagccca caaaggccac caggaagagg
      841 aaggcaccca ctgcaatgat gaccacaggc aacagcgagc cagcagtagt ctcatgggta
      901 attggagctc caccggtttt gtatagttca tccatgccat gtgtaatccc agcagctgtt
      961 acaaactcaa gaaggaccat gtggtctctc ttttcgttgg gatctttcga aagagtagaa
     1021 gttgtaaaca ggtaatggtt gtctgg
//
