LOCUS       sequence-127959-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked kanMX marker, integration into S. cerevisiae XII-2 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2225
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      81..270
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    318..351
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        406..749
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(817..836)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tcataaatca taagaaattc gcttatttag aagtgtcaac aacgtatcta ccaacggaat
       61 gcgtgcgatc gcgtgcattc atccgctcta accgaaaagg aaggagttag acaacctgaa
      121 gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt
      181 caaatttttc ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc
      241 ttgcttgaga aggttttggg acgctcgaag atcgcgtcag ctgaagcttc gtacgctgca
      301 ggtcgacaac ccttaatata acttcgtata atgtatgcta tacgaagtta ttaggtctag
      361 agatctgttt agcttgcctc gtccccgccg ggtcacccgg ccagcgacat ggaggcccag
      421 aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg
      481 tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt tgatggccgc
      541 acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg aaacgctccc
      601 ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat aaaaggttag
      661 gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct aggatacagt
      721 tctcacatca catccgaaca taaacaacca tgggtaagga aaagactcac gtttcgaggc
      781 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
      841 tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt
      901 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
      961 actggctgac ggaatttatg cc
//
