LOCUS       sequence-127961-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked kanMX marker, integration into S. cerevisiae XII-2 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2225
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..62
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     46..62
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    532..565
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 cctgacggat ggcctttttg cgtttctaca aactctttct gtgttgtaaa acgacggcca
       61 gtcttaagct cgggccccct gggcggttct gataacgagt aatcgttaat ccgcaaataa
      121 cgtaaaaacc cgcttcggcg ggttttttta tggggggagt ttagggaaag agcatttgtc
      181 agaatattta agggcgcctg tcactttgct tgatatatga gaattattta accttataaa
      241 tgagaaaaaa gcaacgcact ttaaataaga tacgttgctt tttcgattga tgaacaccta
      301 taattaaact attcatctat tatttatgat tttttgtata tacaatattt ctagtttgtt
      361 aaagagaatt aagaaaataa atctcgaaaa taataaaggg aaaatcagtt tttgatatca
      421 aaattataca tgtcaacgat aatacaaaat ataatacaaa ctataagatg ttatcagtat
      481 ttattatgca tttagaataa attttgtgtc gcccttattc gactcactat agaagttcct
      541 attctctaga aagtatagga acttcacttc attttattta aatttgcggc cgcgctgagg
      601 tcttaatgag cgaacgtaag agaggttaat gtcctctaac tatcagtcca atgacagtat
      661 tttctccttc tcacaaacta caataaatat ctaccaatct cttttcgaat aaggactaat
      721 gatatacgct agttcctgtt tggtaaatga aggctagtgt tcgagaatgg agaacttcgt
      781 agtcggttaa aacagctgta gtgttctggc cttcttagaa agaataaata gttcaggagt
      841 aaaatcaaaa tatcttttaa tataaact
//
