LOCUS       sequence-127963-         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EasyClone system-based yeast integrative vector carrying 
            loxP-flanked kanMX marker, integration into S. cerevisiae XII-2 
            chromosomal location, USER site for cloning, amp resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Stovicek V, Borja GM, Forster J, Borodina I
  TITLE     EasyClone 2.0: expanded toolkit of integrative vectors for stable 
            gene expression in industrial Saccharomyces cerevisiae strains.
  JOURNAL   J Ind Microbiol Biotechnol. 2015 Sep 16.
  PUBMED    26376869
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Ind 
            Microbiol Biotechnol. 2015 Sep 16."
COMMENT     Sequence Label: pCfB2225
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    165..198
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggactc cccatgcgag
       61 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      121 gcccgggcta attatggggt gtcgccctta ttcgactcta tagtgaagtt cctattctct
      181 agaaagtata ggaacttctg aagtggggat ttaaatgcgg ccgcgctgag ggtttaatcg
      241 gcatgcaaac atctacacaa ttagcaaggg caatccatat tttgtctttt cgcgccctgg
      301 aaaggcctaa gtaatgtcgt aaacgcattc tatctgtact tcaactctcc tctgtgcatt
      361 ggtttgtgca aatcacattt tacgatactg ccagatatat gcaaaaagag aaaaccaagg
      421 gaccagaaca aagcaaaatt acgatattct tcgaattcct tcgtgcttga ctaagacaaa
      481 gggatggacg tagcgatttt tagcgggcca agaactggtt ccgaaaaagc acaggtacac
      541 cgaaccctca gctaaggagg gacagcaccg atgcggaagg acaaactttc tttttgccta
      601 tcacagtatc ttatcgagct aactattttc gacacacatg aaaaagcaga aatattaacg
      661 aaaaagaaaa gaaagaccat gtcatgtacg ggcaatcaga atctgtaaca agcgccattt
      721 ttttttctgt atcgggccct ccttactgct ctccttccgt gtaacgcgtt atgattaagt
      781 cctcagcgag ctcgcatgga atgcgtgcga tgagcgacct catgctatac ctgagaaagc
      841 aacctgacct acaggaaaga gttactcaag aataagaatt ttcgttttaa aacctaagag
      901 tc
//
