LOCUS       sequence-127966-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SQ171 fast growing strain supported by pRibo-T plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Orelle C, Carlson ED, Szal T, Florin T, Jewett MC, Mankin AS
  TITLE     Protein synthesis by ribosomes with tethered subunits.
  JOURNAL   Nature. 2015 Jul 29. doi: 10.1038/nature14862.
  PUBMED    26222032
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Jul 29. doi: 10.1038/nature14862."
COMMENT     Sequence Label: SQ171fg/pRibo-T
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             462..470
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             523..537
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="MNHKV"
ORIGIN
        1 ttcggcgttg taagcttaag ctcacaaccc gaagatgttt ctttcgattc atcatcgtgt
       61 tgcgaaaatt tgagagactc acgaacaact ctcgttgttc agtgtttcaa ttttcagctt
      121 gatccagatt tttaaagagc aaatatctca aacatcaccc gaagatgagt tttgagatat
      181 taaggtcggc gactttcact cacaaaccag caagtggcgt cccctagggg attcgaaccc
      241 ctgttaccgc cgtgaaaggg cggtgtcctg ggcctctaga cgaaggggac acgaaaattg
      301 cttatcacgc gttgcgtgat attttcgtgt agggtgagct ttcattaata gaaagcgaac
      361 ggccttattc tcttcagcct cactcccaac gcgtaaacgc cttgcttttc actttctatc
      421 agacaatctg tgtgagcact acaaagtacg cttctttaag gtaaggaggt gatccaaccg
      481 caggttcccc tacggttacc ttgttacgac ttcaccccag tcatgaatca caaagtggta
      541 agcgccctcc tttttttttc tgcgcttaca cacccggcct atcaacgtcg tcgtcttcaa
      601 cgttccttca ggacccttaa agggtcaggg agaactcatc tcggggcaag tttcgtgctt
      661 agatgctttc agcacttatc tcttccgcat ttagctaccg gg
//
