LOCUS       sequence-127977-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a 
            level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pTAtUbq3 (GB0219)
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(657..675)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(658..675)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(693..709)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(693..709)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(706..728)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    717..733
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(741..747,748..765,766..771))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    785..806
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctccggccgc catggcggcc gcgggaattc gattgggcga tgagtggtct cgagcgtgtg
       61 gtaatcacac tattgcacac aaagagtggc aaacaagcgg cgcccttggt ttggtatagt
      121 aagagacaat tgttttggac tgtttgtgtt gccatattca caaaaatact attgagctat
      181 gtatgttaaa cattatggtc aatgagtagt agtgaacatt caagtttttt ttttttccac
      241 ttcaagtttt tcatcttatt attatttttc gttgtgatgc agtagtacgg cccatcttca
      301 ccagtagtat atcaaagccg aaacagatga caaattgttt aacatgcact tggattatta
      361 ttgagagttg aaatacaaag gcccgttaca agcccaaacg aaggtacata aaagaaaagc
      421 agaaaatgaa aacgaaatcg atagggataa gtttttccaa ctgacggaaa cacaaactac
      481 accaggtgaa atagatcacc tcccaagtgc atgagacacg aaccaaccac ttatcatcag
      541 atcacaaaaa gcttaagccg agaccactca tcgcccaatc actagtgaat tcgcggccgc
      601 ctgcaggtcg accatatggg agagctccca acgcgttgga tgcatagctt gagtattcta
      661 tagtgtcacc taaatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
      721 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      781 gcctatgagt gagctaactc acattaattg cgttgcgctc actgcccgct t
//
