LOCUS       sequence-127979-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Chaudhry AZ, Vitullo AD, Gronostajski RM
  TITLE     Nuclear factor I (NFI) isoforms differentially activate simple 
            versus complex NFI-responsive promoters.
  JOURNAL   J Biol Chem. 1998 Jul 17. 273(29):18538-46.
  PUBMED    9660824
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1998 Jul 17. 273(29):18538-46."
COMMENT     Sequence Label: pCHNFI-A4
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..28
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          145..241
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             307..333
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
       61 ccatagaaga caccgggacc gatccagcct ccggactcta gaggatccgg tactcgagga
      121 actgaaaaac cagaaagtta actggtaagt ttagtctttt tgtcttttat ttcaggtccc
      181 ggatccggtg gtggtgcaaa tcaaagaact gctcctcagt ggatgttgcc tttacttcta
      241 ggcctgtacg gaagtgttac ttctgctcta aaagctgcgg aattgtaccc gcggccgcca
      301 gccatgtatc cttacgatgt accagactat gctgggcctc acgggcctcc gctctgtctc
      361 cccaggatga gtttcatcct ttcattgaag cacttctgcc ccatgtccgc gccttcgcct
      421 acacatggtt caacctgcag gcccgaaagc ggaaatactt caaaaaacat gagaagcgca
      481 tgtcgaaaga agaggagagg gccgtgaagg atgaactgct aagcgagaag cccgaggtca
      541 agcagaagtg ggcttcccga cttctggcca agttacggaa agatatccga cccgagtacc
      601 gagaggattt tgttcttaca gttacaggga aaaaacctcc atgctgtgtt ctttccaacc
      661 ctgatcagaa aggcaagatg cggagaattg actgcctccg ccaggcagat aaagtatgga
      721 ggttggacct cgtcatggtg atcttgttca aaggtattcc gctggaaagt actgatggcg
      781 agcgccttgt aaagagtcca cagtgctcta atccagggct ctgtgtccag ccccatcaca
      841 taggggtttc tgtaaaggaa ctcgatttat atttggcata ctttgtacat gcagcagatt
      901 caagtcaatc tgaaagtccc agccagccaa gtgaagctga catta
//
