LOCUS       sequence-127988-         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-Q10
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(26..57)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 cggggcgtaa acgccatggg catgtagtca aaagcctccg gtcggaggct tttgacttgg
       61 ctgaggagtg ccgttaatta agttcagagc tggaagttac tcaaatcgtc ggtgtccact
      121 tccgagtcaa tctgcccgac cagataagaa ctgacttcca cttcctgcgg agcaacctgc
      181 acgttatcag acaccagcca agtgttgatc cacgggatcg ggttggagcg cgtctggaac
      241 ggcagatcca aaccgactgc ctgcatacgg atattggtga tgtattcaac gtactggcag
      301 agaatgtctt tattcagacc aatcatcgaa ccgtcgcgga acagataatc cgcccagtct
      361 ttctcctgtt gagctgcctg aacaaacagg tcatagcact cctgcttaca ctcttcggca
      421 atttccgcca tctcaggatc gtccgcgccg ctgcgcagca gattcagcat atgctgggtg
      481 ccggtcaggt gcagggcttc gtcgcgggca atcaggcgaa taattttggc gttgccttcc
      541 atcaattcgc gttctgcaaa tgcgaaggaa caagcaaagc tgacgtagaa acgaatcgct
      601 tccagcgcgt taacgctcat caggcagaga tacagttttt tcttcagctc gcgcaggcta
      661 acggtcacag ttttaccgtt aacggtgtgg gtaccttcgc c
//
