LOCUS       sequence-127994-         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes a pH-sensitive ratiometric GFP (pH-GFP), which allows for 
            noninvasive measurements of intrabacterial pH in live cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Vandal OH, Pierini LM, Schnappinger D, Nathan CF, Ehrt S
  TITLE     A membrane protein preserves intrabacterial pH in intraphagosomal 
            Mycobacterium tuberculosis.
  JOURNAL   Nat Med. 2008 Aug;14(8):849-54. doi: 10.1038/nm.1795. Epub 2008 Jul 
            20.
  PUBMED    18641659
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nm.1795"; 
            journalName: "Nat Med"; date: "2008-08"; volume: "14"; issue: "8"; 
            pages: "849-54"
COMMENT     Sequence Label: pUV15-pHGFP
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(67..87)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 attagctaaa gcttgatatc accttatttg tatagttcat ccatgccatg tgtaatccca
       61 gcagctgtta caaactcaag aaggaccatg tggtctctct tttcgttggg atctttcgaa
      121 agggcagatt gtgtgtgcag gtaatggttg tctggtaaaa ggacagggcc atcgccaatt
      181 ggagtatttt gttgataatg gtctgctagt tgaacgcctc catcttcaat gttgtggtga
      241 acttgaaaga tagctttggt accattcttt tgtttgtctg ccatgatgta caccaagtgc
      301 tcgttatagt tgtattccaa tttgtgtcca agaatgtttc catcatcttt aaaatcaata
      361 ccttttaact cgattctatt aacaagggta tcaccttcaa acttgacttc agcacgtgtc
      421 ttgtagttcc cgtcatcttt gaaaaatata gttctttcct gtacataacc ttcnggcatg
      481 gcactcttga aaaagtcatg ccgtttcata tgatctgggt atcttgaaaa gcattgaaca
      541 ccataagaga aagtagtgac aagtgttggc catggaacag gtagttttcc antagtgcaa
      601 ataaatttaa gggtaagttt tccgtatgtt gcatcacctt caccctctcc actgacagaa
      661 aatttgtgcc cattaacatc accatctaat tcaacaagaa ttg
//
