LOCUS       sequence-127996-         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-S
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(16..47)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 acgccatggg catgtagtca aaagcctccg gtcggaggct tttgacttgg ctgaggagtg
       61 ccgttaatta agtttaagat ttaacgaagt tttcttccgc tttcatcatc gcgtcggtgt
      121 tggtagacgc cagacgccac aggatgaaca tgaaaacaga acggtacgga gagaaggttt
      181 cagagcattt ttccatgatg tcgtcgtcgt agattttcca gttgtatttt ttgtgtttga
      241 ttttagattt tttaacaact ttacgttcac gcatcagttc tttttccagt tccggtttgt
      301 cagacaggta tttagagaaa ccacgcgcga tacccaggtc ttccggcgcg aaaacgtcca
      361 tacgtttcag accagagatc aggaacattt tcgcagacca cggaccgata cctttaacgt
      421 tggtaaccag agattcgata acttcttcgt cgttgtcttt ctgaccgaac agtttttcga
      481 tgtctttgta tttttcggtg aagtaaaccg ccagagattc caggtagatc attttacgtt
      541 tagacagacc gcatttcgcg atttccgcgc atttcgccgg gtctttgaag tcttcgaaca
      601 ggattttgta gtccgggaac gcaccaccgt a
//
