LOCUS       sequence-127997-         625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoterless AAV plasmid with MCS to insert promoter for expression 
            of EmGFP. Contains WPRE..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS2157
FEATURES             Location/Qualifiers
     source          1..625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(19..40)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     368..387
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
       61 gtctttgctc agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc
      121 gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat
      181 gttgtggcgg gtcttgaagt tcaccttgat gccgttcttc tgcttgtcgg cggtgatata
      241 gaccttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt
      301 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc
      361 ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc
      421 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcgggcgaa
      481 gcactgcacg ccgtangtga aggtggtcac gagggtgggc cagggcacgg gcagcttgcc
      541 ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
      601 ggacacgctg aacttgtggc cgttt
//
