LOCUS       sequence-128005-         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual-promoter plasmid to express knockdown-proof human ch-TOG with 
            L1939A and L1942A substitutions tagged with EGFP along with shRNA to
            knock down endogenous ch-TOG in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Gutierrez-Caballero C, Burgess SG, Bayliss R, Royle SJ
  TITLE     TACC3-ch-TOG track the growing tips of microtubules independently of
            clathrin and Aurora-A phosphorylation.
  JOURNAL   Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843.
  PUBMED    25596274
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/bio"; 
            journalName: "Biol Open"; date: "2015-01-16- 16"; volume: "4"; 
            issue: "2"; pages: "170-9"
COMMENT     Sequence Label: pBrain-ch-TOGKDP(L1939A,L1942A)-GFP-shch-TOG
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(91..107)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(92..108)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(136..154)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(175..191)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(175..191)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(188..210)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    199..215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(223..229,230..247,248..253))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    268..289
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     enhancer        392..695
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 agttctgtat gagaccacag atcccctgct ccacagcaaa ctctcttcaa gagagagagt
       61 ttgctgtgga gcatttttgg aaaagcttat cgataccgtc gacctcgagg gggggcccgg
      121 taccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata
      181 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      241 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      301 ctcactgccc gctttccagt cgggaaacct gtcgtgccag atagtaatca attacggggt
      361 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      421 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      481 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      541 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      601 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      661 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      721 atgg
//
