LOCUS       sequence-128009-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG305GAL-ccdB-DsRed
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(205..309)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     377..395
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(433..455)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     555..574
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
       61 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
      121 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
      181 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
      241 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
      301 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
      361 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
      421 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
      481 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
      541 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tcgactacgt
      601 cgttaaggcc gtttctgaca gagtaaaatt cttgagggaa ctttcaccat tatgggaaat
      661 ggttcaagaa ggtattgact taaactccat caaatggtca ggtcattgag tgttttttat
      721 ttgttgtatt tttttttttt tagagaaaat cctccaatat ataaattagg aatcatagtt
      781 tcatgatttt ctgttacacc taactttttg tgtggtgccc tcctccttgt caatattaat
      841 gttaaagtgc aattcttttt ccttatcacg ttgagccatt agtatcaatt tgcttacctg
      901 tattccttta catcctcctt tttctccttc ttgataaatg tatgtagatt gcgtatatag
      961 tttcgtctac cctatgaaca tattccattt tgtaatttcg tgtcgtttct attatgaatt
     1021 tcatttataa agtt
//
