LOCUS       sequence-128011-         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG305GAL-Cerulean-ccdB
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(192..296)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     364..382
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(420..442)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     542..561
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
       61 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      121 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
      181 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
      241 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
      301 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
      361 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
      421 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
      481 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
      541 gcggcatcag agcagattgt actgagagtg caccatatcg actacgtcgt taaggccgtt
      601 tctgacagag taaaattctt gagggaactt tcaccattat gggaaatggt tcaagaaggt
      661 attgacttaa actccatcaa atggtcaggt cattgagtgt tttttatttg ttgtattt
//
