LOCUS       sequence-128012-         567 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG305GAL-Cerulean-ccdB
FEATURES             Location/Qualifiers
     source          1..567
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..395
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(51..70)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
       61 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
      121 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
      181 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
      241 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
      301 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
      361 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
      421 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
      481 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
      541 gagcgtgggt ctcgcggtat cattgca
//
