LOCUS       sequence-128013-         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG305GPD-ccdB-DsRed
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(193..297)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     365..383
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(421..443)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     543..562
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
       61 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
      121 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
      181 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
      241 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
      301 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
      361 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
      421 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
      481 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
      541 tgcggcatca gagcagattg tactgagagt gcaccatatc gactacgtcg ttaaggccgt
      601 ttctgacaga gtaaaattct tgagggaact ttcaccatta tgggaaatgg ttcaagaagg
      661 tattgactta aactccatca aatggtcagg tcattgagtg ttttttattt gttgtattt
//
