LOCUS       sequence-128016-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET-based biosensor reporting on endogenous Cdc42 activation (for 
            Lentivirus production).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Martin K, Reimann A, Fritz RD, Ryu H, Jeon NL, Pertz O
  TITLE     Spatio-temporal co-ordination of RhoA, Rac1 and Cdc42 activation 
            during prototypical edge protrusion and retraction dynamics.
  JOURNAL   Sci Rep. 2016 Feb 25;6:21901. doi: 10.1038/srep21901.
  PUBMED    26912264
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-25"; volume: "6:21901. doi"; pages: " 
            10.1038/srep21901"
COMMENT     Sequence Label: pLenti-Cdc42-2G
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(66..83)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgac gaattcatat gctagctcta gactgcagga
       61 tcctggtgat ggtgatggtg gtgctcgagt catagcagca cacacctgcg gctcttcttc
      121 ggttctggag gctccagggc agccaatatt gcttcatcaa acacattctt taggcctttc
      181 tgtgtgaggg cagagcactc cacatacttg acagccttca gatcccgcgc cagcttttca
      241 gcagtctctg gagtaatagg cttctgtttg ttcttggcaa gtttctcaat agtagagggg
      301 tcatctctga gatcaatttg ggtcccaaca agcaagaaag gagtctttgg acagtggtga
      361 gttatctcag gcacccactt ttctttcaca ttttcaaatg aggatggaga gaccactgag
      421 aaacatacta gaaaaacatc tgtctgtgga taacttagcg gtcgtagtct gtcataatcc
      481 tcttgccctg cagtatcaaa aagtccaaga gtgtatggct ctccaccaat cataactgtg
      541 actgcatant tgtcaaaaac agttggtaca tattccgatg ggaatttgtt tgttgtgtan
      601 gatatcanga gacatgtttt accaacagca ccatcaccaa caacaacaca cttaattgtc
      661 tgcatggtac ccacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
      721 tgcacgccgc cgtcctcgat gttgtggcgg
//
