LOCUS       sequence-128020-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of lyso-pHoenix in mammalian cells for optogenetic 
            acidification of lysosomes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Rost BR, Schneider F, Grauel MK, Wozny C, G Bentz C, Blessing A, 
            Rosenmund T, Jentsch TJ, Schmitz D, Hegemann P, Rosenmund C
  TITLE     Optogenetic acidification of synaptic vesicles and lysosomes.
  JOURNAL   Nat Neurosci. 2015 Nov 9. doi: 10.1038/nn.4161.
  PUBMED    26551543
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 Nov 9. doi: 10.1038/nn.4161."
COMMENT     Sequence Label: pCMV-lyso-pHoenix
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..806
                     /codon_start=1
                     /product="light-driven proton pump archaerhodopsin-3 from 
                     Halorubrum sodomense (Chow et al., 2010)"
                     /label=Arch
                     /translation="MDPIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT
                     DKDAREYYAVTILVPGIASAAYLSMFFGIGLTEVTVGGEMLDIYYARYADWLFTTPLLL
                     LDLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYFLA
                     TSLRSAAKERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD
                     VTAKVGFGFILLRSRAILGDTEAPEPSAGADVSAAD"
ORIGIN
        1 tggatgaact atacaaaacc ggtaccggat ccatggaccc catcgctctg caggctggtt
       61 acgacctgct gggtgacggc agacctgaaa ctctgtggct gggcatcggc actctgctga
      121 tgctgattgg aaccttctac tttctggtcc gcggatgggg agtcaccgat aaggatgccc
      181 gggaatatta cgctgtgact atcctggtgc ccggaatcgc atccgccgca tatctgtcta
      241 tgttctttgg tatcgggctt actgaggtga ccgtcggggg cgaaatgttg gatatctatt
      301 atgccaggta cgccgactgg ctgtttacca ccccacttct gctgctggat ctggcccttc
      361 tcgctaaggt ggatcgggtg accatcggca ccctggtggg tgtggacgcc ctgatgatcg
      421 tcactggcct catcggagcc ttgagccaca cggccatagc cagatacagt tggtggttgt
      481 tctctacaat ttgcatgata gtggtgctct attttctggc tacatccctg cgatctgctg
      541 caaaggagcg gggccccgag gtggcatcta cctttaacac cctgacagct ctggtcttgg
      601 tgctgtggac cgcttaccct atcctgtgga tcataggcac tgagggcgct ggcgtggtgg
      661 gcctgggcat cgaaactctg ctgtttatgg tgttggacgt gactgccaag gtcggctttg
      721 gctttatcct gttgagatcc cgggctattc tgggcgacac cgaggcacca gaacccagtg
      781 ccggtgccga tgtcagtgcc gccgaccgta cgatggtgag cgagctgatt aaggagaaca
      841 tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg
      901 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggcg gtcgagggcg
      961 gccctctccc cttcgcctt
//
