LOCUS       sequence-128021-        1116 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of lyso-pHoenix in mammalian cells for optogenetic 
            acidification of lysosomes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Rost BR, Schneider F, Grauel MK, Wozny C, G Bentz C, Blessing A, 
            Rosenmund T, Jentsch TJ, Schmitz D, Hegemann P, Rosenmund C
  TITLE     Optogenetic acidification of synaptic vesicles and lysosomes.
  JOURNAL   Nat Neurosci. 2015 Nov 9. doi: 10.1038/nn.4161.
  PUBMED    26551543
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 Nov 9. doi: 10.1038/nn.4161."
COMMENT     Sequence Label: pCMV-lyso-pHoenix
FEATURES             Location/Qualifiers
     source          1..1116
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             983..1009
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(1080..1099)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ggtggctgtg gccagatact gcgacctccc tagcaaactg gggcacagaa agcttgcagc
       61 cctgcaggag aagaagtcat gcagccagcg catggccgaa ttccggcaat actgttggaa
      121 cccggacact gggcagatgc tgggccgcac cccagcccgg tgggtgtgga tcagcctgta
      181 ctatgcagct ttctacgtgg tcatgactgg gctctttgcc ttgtgcatct atgtgctgat
      241 gcagaccatt gatccctaca cccccgacta ccaggaccag ttaaagtcac cgggggtaac
      301 cttgagaccg gatgtgtatg gggaaagagg gctgcagatt tcctacaaca tctctgaaaa
      361 cagctctggt ttaaacacca ccggtggagc tccaattacc catgagacta ctgctggctc
      421 gctgttgcct gtggtcatca ttgcagtggg tgccttcctc ttcctggtgg cctttgtggg
      481 ctgctgtggg gcctgcaagg agaactactg tctcatgatt acatttgcca tcttcctgtc
      541 tcttatcatg cttgtggagg tggctgtggc cattgctggc tatgtgttta gagaccaggt
      601 gaagtcagag tttaataaaa gcttccagca gcagatgcag aattacctta aagacaacaa
      661 aacagccact attttggaca aattgcagaa agaaaataac tgctgtggag cttctaacta
      721 cacagactgg gaaaacatcc ccggcatggc caaggacaga gtccccgatt cttgctgcat
      781 caacataact gtgggctgtg ggaatgattt caaggaatcc actatccata cccagggctg
      841 cgtggagact atagcaatat ggctaaggaa gaacatactg ctggtggctg cagcggccct
      901 gggcattgct tttgtggagg tcttgggaat tatcttctcc tgctgtctgg tgaagagtat
      961 tcgaagtggc tatgaagtaa tgtacccata cgatgttcca gattacgctt agttaattaa
     1021 gcaagggcga attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     1081 caatagcatc acaaatttca caaataaagc attttt
//
