LOCUS       sequence-128029-         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses phosphomutant mouse ATP-citrate lyase in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Lee JV, Carrer A, Shah S, Snyder NW, Wei S, Venneti S, Worth AJ, 
            Yuan ZF, Lim HW, Liu S, Jackson E, Aiello NM, Haas NB, Rebbeck TR, 
            Judkins A, Won KJ, Chodosh LA, Garcia BA, Stanger BZ, Feldman MD, 
            Blair IA, Wellen KE
  TITLE     Akt-dependent metabolic reprogramming regulates tumor cell histone 
            acetylation.
  JOURNAL   Cell Metab. 2014 Aug 5;20(2):306-19. doi: 
            10.1016/j.cmet.2014.06.004. Epub 2014 Jul 3.
  PUBMED    24998913
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2014.06.004"; journalName: "Cell Metab"; date: 
            "2014-08-5- 5"; volume: "20"; issue: "2"; pages: "306-19"
COMMENT     Sequence Label: pEF6-Acly(S455A)
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             290..319
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             398..439
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             449..466
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(489..506)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    495..606
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctatgccctg gaagtggaga agattaccac ctccaagaag ccaaatctta tcctgaatgt
       61 ggacggcttc atcggcgttg cgtttgtgga catgctcagg aactgtggct ccttcacccg
      121 ggaggaagct gatgaatatg ttgacattgg agccctcaat ggcatctttg tgctaggaag
      181 gagtatgggc ttcattgggc actaccttga ccagaagagg ctgaagcaag ggctgtatcg
      241 tcacccctgg gatgacattt cctatgttct tccagaacac atgagcatgg aacaaaaact
      301 catctcagaa gaggatctgt gaattcgggg gaagggcaat tctgcagata tccagcacag
      361 tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct
      421 cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacccg
      481 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
      541 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
      601 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
      661 caaggg
//
