LOCUS       sequence-128033-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TpR mini-Tn7 vector, mobilizable, for CFP tagging bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Tp-ecfp
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      48..82
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     terminator      195..281
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    332..379
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(441..677)
                     /codon_start=1
                     /gene="dhfrIIb from plasmid R388"
                     /product="E. coli plasmid-associated dihydrofolate 
                     reductase"
                     /label=TpR
                     /note="confers resistance to trimethoprim"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
ORIGIN
        1 tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg
       61 gttgccgccg ggcgtttttt attggtgaga atccaagcta gactgcgatg agtggcaggg
      121 cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctgggg taatgactct
      181 ctagcttgag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      241 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgcta ggagcttgcg
      301 gcccggacga tgagctcgaa ttggggatct tgaagtacct attccgaagt tcctattctc
      361 tagaaagtat aggaacttca gagcgctttt gaagctgatg tgcttaaaaa cttactcaat
      421 ggaataattc tagataattc ttaggccaca cgttcaagtg cagccacagg ataaatttgc
      481 actgagcctg ggtgggattc ggactcgacc gcatagcctt caggagtgag ttttgtgcaa
      541 taccaaccga cgacttgacc ctgccaagcg gcaccagatt tcttgcgtac gcgatcccct
      601 aagccaaagg tggcactcag gggaagcgca aactgccctg caacgggagc gttggcttca
      661 tcgctacttt gacccatgtc gaatccttct tgtgaatcta ttatggcgac aagcaaatcg
      721 agctctgact gcctacccca caa
//
