LOCUS       sequence-128040-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the CDS of the Antirrhinum majus Rosea transcription factor
            as a level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pRosea (GB0026)
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(819..837)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(820..837)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(855..871)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(855..871)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctccggccgc catggcggcc gcgggaattc gattgggcga tgagtggtct cgaatggaaa
       61 agaattgtcg tggagtgaga aaaggtactt ggaccaaaga agaagacact ctcttgaggc
      121 aatgtataga agagtatggt gaagggaaat ggcatcaagt tccacacaga gcagggttga
      181 accggtgtag gaagagttgc aggctgaggt ggttgaatta tctgaggcca aatatcaaaa
      241 gaggtcggtt ttcgagagat gaagtggacc taattgtgag gcttcataag ctgttgggta
      301 acaaatggtc gctgattgct ggtagaattc ctggaaggac agctaatgac gtgaagaact
      361 tttggaatac tcatgtgggg aagaatttag gcgaggatgg agaacgatgc cggaaaaatg
      421 ttatgaacac aaaaaccatt aagctgacta atatcgtaag accccgagct cggaccttca
      481 ccggattgca cgttacttgg ccgagagaag tcggaaaaac cgatgaattt tcaaatgtcc
      541 ggttaacaac tgatgagatt ccagattgtg agaagcaaac gcaattttac aatgatgttg
      601 cgtcgccaca agatgaagtt gaagactgca ttcagtggtg gagtaagttg ctagaaacaa
      661 cggaggatgg ggaattagga aacctattcg aggaggccca acaaattgga aattgagctt
      721 cgagaccact catcgcccaa tcactagtga attcgcggcc gcctgcaggt cgaccatatg
      781 ggagagctcc caacgcgttg gatgcatagc ttgagtattc tatagtgtca cctaaatagc
      841 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatcc
//
