LOCUS       sequence-128047-        6141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene.
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pBabe-GFP-IKBalpha-mut (super repressor)
FEATURES             Location/Qualifiers
     source          1..6141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             942..1358
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1288..1310
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1326..1342
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2374..2394)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2379..2708
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2559..2694
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2717..2733)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2718..2734)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             2739..3458
                     /codon_start=1
                     /product="humanized Renilla green fluorescent protein"
                     /label=hrGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN
                     QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE
                     DGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG
                     QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI
                     AQLTSLGKPLGSLHEWV"
     LTR             3635..4104
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4234..4251)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4405..4993)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4485..4504)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5153..5944,5945..6013))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5776..5795
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6014..6118)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatctct cgaggttagc
     1381 ttatgttcca ggcggccgag cgtccccagg agtgggccat ggagggcccc cgcgacgggc
     1441 tgaagaagga gcggctactg gacgaccgcc acgacgccgg cctggacgcc atgaaagacg
     1501 aggagtacga gcagatggtc aaggagctgc aggagatccg cctcgagccg caggaggtgc
     1561 cgcgcggctc ggagccctgg aagcagcagc tcaccgagga cggggactcg ttcctgcact
     1621 tggccatcat ccatgaagaa aaggcactga ccatggaagt gatccgccag gtgaagggag
     1681 acctggcctt cctcaacttc cagaacaacc tgcagcagac tccactccac ttggctgtga
     1741 tcaccaacca gccagaaatt gctgaggcac ttctgggagc tggctgtgat cctgagctcc
     1801 gagactttcg aggaaatacc cccctacacc ttgcctgtga gcagggctgc ctggccagcg
     1861 tgggagtcct gactcagtcc tgcaccaccc cgcacctcca ctccatcctg aaggctacca
     1921 actacaatgg ccacacgtgt ctacacttag cctctatcca tggctacctg ggcatcgtgg
     1981 agcttttggt gtccttgggt gctgatgtca atgctcagga gccctgtaat ggccggactg
     2041 cccttcacct cgcagtggac ctgcaaaatc ctgacctggt gtcactcctg ttgaagtgtg
     2101 gggctgatgt caacagagtt acctaccagg gctattctcc ctaccagctc acctggggcc
     2161 gcccaagcac ccggatacag cagcagctgg gccagctgac actagaaaac cttcagatgc
     2221 tgccagagag tgaggatgag gagagctatg acacagagtc agagttcacg gagttcacag
     2281 aggacgagct gccctatgat gactgtgtgt ttggaggcca gcgtctgacg ttatgaggat
     2341 caacgaattc gccagcacag tggtcgaccc tgtggaatgt gtgtcagtta gggtgtggaa
     2401 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     2461 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     2521 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     2581 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     2641 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     2701 tttgcaaaaa gcttatcgat accgtcgacc tcgagaccat ggtgagcaag cagatcctga
     2761 agaacaccgg cctgcaggag atcatgagct tcaaggtgaa cctggagggc gtggtgaaca
     2821 accacgtgtt caccatggag ggctgcggca agggcaacat cctgttcggc aaccagctgg
     2881 tgcagatccg cgtgaccaag ggcgcccccc tgcccttcgc cttcgacatc ctgagccccg
     2941 ccttccagta cggcaaccgc accttcacca agtaccccga ggacatcagc gacttcttca
     3001 tccagagctt ccccgccggc ttcgtgtacg agcgcaccct gcgctacgag gacggcggcc
     3061 tggtggagat ccgcagcgac atcaacctga tcgaggagat gttcgtgtac cgcgtggagt
     3121 acaagggccg caacttcccc aacgacggcc ccgtgatgaa gaagaccatc accggcctgc
     3181 agcccagctt cgaggtggtg tacatgaacg acggcgtgct ggtgggccag gtgatcctgg
     3241 tgtaccgcct gaacagcggc aagttctaca gctgccacat gcgcaccctg atgaagagca
     3301 agggcgtggt gaaggacttc cccgagtacc acttcatcca gcaccgcctg gagaagacct
     3361 acgtggagga cggcggcttc gtggagcagc acgagaccgc catcgcccag ctgaccagcc
     3421 tgggcaagcc cctgggcagc ctgcacgagt gggtgtaata gggtaccttc gataaaataa
     3481 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
     3541 aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag
     3601 aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct
     3661 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg
     3721 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt
     3781 ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc
     3841 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
     3901 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
     3961 ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt
     4021 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
     4081 ctacccgtca gcgggggtct ttcacatgca gcatgtatca aaattaattt ggtttttttt
     4141 cttaagtatt tacattaaat ggccatagtt gcattaatga atcggccaac gcgcggggag
     4201 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     4261 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     4321 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4381 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4441 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4501 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4561 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4621 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4681 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4741 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4801 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     4861 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4921 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4981 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5041 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5101 tttgcggccg caaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     5161 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5221 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     5281 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     5341 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5401 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5461 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5521 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5581 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5641 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5701 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5761 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5821 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5881 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5941 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     6001 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     6061 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     6121 catttccccg aaaagtgcca c
//
