LOCUS       sequence-128049-         201 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides a Short guide RNA which is the combination of the bacterial
            crRNA and tracrRNA into a single guide transcript; it does not 
            contain any target site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 201)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: psgRNA (GB0645)
FEATURES             Location/Qualifiers
     source          1..201
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(152..176)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctccggccgg ccatggccgg cccgccggga aattcgaatt ggggcgatga gtggctctcg
       61 gttttagacg ctagaaatag caaggttaaa ataaggctag tccgattatc aacttgaaaa
      121 agtggcaccg agatcggtgc tttttttcta gacccagctt tcttgtacaa agttggcatt
      181 acgctcgaga ccactcatcg c
//
