LOCUS       sequence-128052-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the bar gene for resistance to basta fused to the A. 
            tumefaciens Nopaline synthase promoter and terminator. Cloned as a 
            level 0 GB part ready to be transferred to a GB level1 plant 
            expression vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pTNos:BASTA:PNos (GB0023)
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        165..344
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 acgcgttggg agctctccca tatggtcgac ctgcaggcgg ccgcgaattc actagtgatt
       61 gggcgatgag tggtctcgag cgtatcatac atgagaatta agggagtcac gttatgaccc
      121 ccgccgatga cgcgggacaa gccgttttac gtttggaact gacagaaccg caacgttgaa
      181 ggagccactg agccgcgggt ttctggagtt taatgagcta agcacatacg tcagaaacca
      241 ttattgcgcg ttcaaaagtc gcctaaggtc actatcagct agcaaatatt tcttgtcaaa
      301 aatgctccac tgacgttcca taaattcccc tcggtatcca attagagtct catattcact
      361 ctcaactcga tcgaggggat ctaccatgag cccagaacga cgcccggccg acatccgccg
      421 tgccaccgag gcggacatgc cggcggtctg caccatcgtc aaccactaca tcgagacaag
      481 cacggtcaac ttccgtaccg agccgcagga accgcaggag tggacggacg acctcgtccg
      541 tctgcgggag cgctatccct ggctcgtcgc cgaggtggac ggcgaggtcg ccggcatagc
      601 ctacgcgggt ccctggaagg cacgcaacgc ctacgactgg acggccgagt cgaccgtgta
      661 cgtgtccccc cgccaccagc ggacgggact gggctccacg ctctacaccc acctgctgaa
      721 gtccctggag gcacagggct tcaagagcgt ggtcgctgtc atcgggctgc ccaacgaccc
      781 gagcgtgcgc atgcacgagg cgctcggata tgccccccgc ggcatgctgc gggcggccgg
      841 cttcaagcac gggaactggc atgacgtgg
//
