LOCUS       sequence-128053-         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the bar gene for resistance to basta fused to the A. 
            tumefaciens Nopaline synthase promoter and terminator. Cloned as a 
            level 0 GB part ready to be transferred to a GB level1 plant 
            expression vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pTNos:BASTA:PNos (GB0023)
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      complement(76..328)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(348..893)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="PERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQEWT
                     DDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGSTL
                     YTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGFWQ
                     LDFSLPVPPRPVLPVTEI"
ORIGIN
        1 ctccggccgc catggcggcc gcgggaattc gattgggcga tgagtggtct cgggagatca
       61 gcttgcatgc cggtcgatct agtaacatag atgacaccgc gcgcgataat ttatcctagt
      121 ttgcgcgcta tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca
      181 taaaaaccca tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt
      241 aattcaacag aaattatatg ataatcatcc caagaccggc aacaggattc aatcttaaga
      301 aactttattg ccaaatgttt gaacgatctg cttgactcta ggggtcatca gatttcggtg
      361 acgggcagga ccggacgggg cggcaccggc aggctgaagt ccagctgcca gaaacccacg
      421 tcatgccagt tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg ggcatatccg
      481 agcgcctcgt gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc gaccacgctc
      541 ttgaagccct gtgcctccag ggacttcagc aggtgggtgt agagcgtgga gcccagtccc
      601 gtccgctggt ggcgggggga cacgtacacg gtcgactcgg ccgtccagtc gtaggcgttg
      661 cgtgccttcc agggacccgc gtaggctatg ccggcgacct cgccgtccac ctcggcgacg
      721 agccagggat agcgctcccg cagacggacg aggtcgtccg tccactcctg cggttcctgc
      781 ggctcggtac ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat ggtgcagacc
      841 gccggcatgt ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc tgg
//
