LOCUS       sequence-128059-         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GoldenBraid level 1 Alpha plant expression vector. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB1_alpha2
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..41
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(56..61,62..79,80..86)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    94..110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     99..121
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     118..134
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     118..134
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    147..203
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(204..221)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(204..220)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(213..235)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(429..448)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    570..592
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
       61 actatatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
      121 gaaacagcta tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat
      181 ccccgggtac cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
      241 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      301 agcgaagagg cctgcgccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      361 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
      421 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
      481 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
      541 ggtctctctc ctgactgaga cgaagcttgt tacaccacaa tatatcctgc caagatctaa
      601 ttccggggat cggaaatcca gaagcccgag aggttgccgc ctttcgggct ttttcttttt
      661 caaaaaaaaa aatttataaa acgatctgtt gcggccggcc gccgggttgt gggcaaaggc
      721 gctcgacggt gggcaa
//
