LOCUS       sequence-128061-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GoldenBraid level 1 Alpha plant expression vector. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB1_alpha2R
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    18..42
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     161..180
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     374..396
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     388..405
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     389..405
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    406..462
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(475..491)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(475..491)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(488..510)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    499..515
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(523..529,530..547,548..553))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    568..589
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    673..695
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 acacatacag cgacttagtt tacccgccaa tatatcctgt cagatatcgt ctcaagcgga
       61 gagagaccca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
      121 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
      181 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
      241 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggcgcaggcc
      301 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
      361 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt
      421 acccggggat cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata
      481 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      541 catatagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      601 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctggggtctc tcgcttgact
      661 gagacgatat cgttacacca caatatatcc tgccaagatc taattccggg gatcggaaat
      721 ccagaagccc gagaggttgc cgcctttcgg gctttttctt tttcaaaaaa aaaaatttat
      781 aaaacgatct gttgcggccg gccgccgggt tgtgggcaaa ggcgctcgac ggtgg
//
