LOCUS       sequence-128065-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GoldenBraid level 1 Alpha plant expression vector. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB3_alpha1
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    475..499
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     620..639
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tcgcggcctt gagcaagacg tttcccgttg aatatggctc ataacagaac ttattatttc
       61 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact
      121 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
      181 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat
      241 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc
      301 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa
      361 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt
      421 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag
      481 gatatattgt ggtgtaaaca taacgaattc gtctcaggag agagacccac agcttgtctg
      541 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
      601 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
      661 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
      721 aggctgcgca actgttggga agggcgatcg gcgcaggcct cttcgctatt acgccagctg
      781 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
//
