LOCUS       sequence-128077-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GoldenBraid level 1 Alpha plant expression vector. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB3_alpha2R
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    475..499
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    587..608
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(623..628,629..646,647..653)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    661..677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     666..688
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     685..701
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     685..701
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tcgcggcctt gagcaagacg tttcccgttg aatatggctc ataacagaac ttattatttc
       61 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact
      121 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
      181 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat
      241 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc
      301 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa
      361 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt
      421 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag
      481 gatatattgt ggtgtaaaca taacgatatc gtctcagtca agcgagagac cccagctggc
      541 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      601 tcactcatta ggcaccccag gctttacact atatgcttcc ggctcgtatg ttgtgtggaa
      661 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      721 catgcctgca ggtcgactct agaggatccc
//
