LOCUS       sequence-128107-       11052 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and 
            iRFP670.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11052)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 11052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: pGK:TRL-EFS:iRFP670
FEATURES             Location/Qualifiers
     source          1..11052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1685..1918
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2103..2147
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2445..2562
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(2698..2721)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3095..3114
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3190..3810
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MARLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3263..3282)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             3811..3831
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3934..4128
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             4210..4266
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4267..4971
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     CDS             4987..5043
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5044..6696
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(5110..5128)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        6764..6975
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    7941..8529
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7994..8014)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8412..8423)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8532..8548)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8533..8549)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9058..9238
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(9300..9888)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9380..9399)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10059..10850,10851..10919))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10682..10701
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10920..11024)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct ggaggaggag
     1561 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1621 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1681 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1741 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     1801 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1861 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1921 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1981 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     2041 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     2101 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     2161 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     2221 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     2281 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     2341 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     2401 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2461 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2521 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2581 agcagagatc cagtttggtt agtaccgggc ccgggtcgac tagtgatcag tgtgagggag
     2641 tgtaaagctg gtttattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     2701 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     2761 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     2821 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     2881 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     2941 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     3001 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
     3061 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
     3121 tctgccgcgc tgttctcctc ttcctcatct ccgggactaa ctcgaacgct agctgtgcga
     3181 tcgtttacca tggctagatt agataaaagt aaagtgatta acagcgcatt agagctgctt
     3241 aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag
     3301 cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt
     3361 gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag ctggcaagat
     3421 tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg cgatggagca
     3481 aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga aaatcaatta
     3541 gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact cagcgctgtg
     3601 gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc taaagaagaa
     3661 agggaaacac ctactactga tagtatgccg ccattattac gacaagctat cgaattattt
     3721 gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat atgcggatta
     3781 gaaaaacaac ttaaatgtga aagtgggtcg ccaaaaaaga agagaaaggt cgacggcggt
     3841 ggtgctttgt ctcctcagca ctctgctgtc actcaaggaa gtatcatcaa gaacaaggag
     3901 ggcatggatg ctaagtcact aactgcctgg tcccggacac tggtgacctt caaggatgta
     3961 tttgtggact tcaccaggga ggagtggaag ctgctggaca ctgctcagca gatcgtgtac
     4021 agaaatgtga tgctggagaa ctataagaac ctggtttcct tgggttatca gcttactaag
     4081 ccagatgtga tcctccggtt ggagaaggga gaagagccct ggctggtgga gagagaaatt
     4141 caccaagaga cccatcctga ttcagagact gcatttgaaa tcaaatcatc agttaaactg
     4201 ggcagcggtg ccactaactt cagcttgttg aagcaggccg gagacgtcga agagaacccg
     4261 ggtccaatgt ctagactgga caagagcaaa gtcataaacg gagctctgga attactcaat
     4321 ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag
     4381 cctaccctgt actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag
     4441 atgctggaca ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt
     4501 ctgcggaaca acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa
     4561 gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg
     4621 ttcctgtgtc agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc
     4681 cactttacac tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga
     4741 gagacaccta ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac
     4801 cggcagggag ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag
     4861 aaacagctaa agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac
     4921 atgctcccag ccgatgccct tgacgatttt gaccttgaca tgctccccgg gaagttagga
     4981 tccggagcca cgaacttctc tctgttaaag caagcaggag acgtggaaga aaaccccggg
     5041 cccatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat
     5101 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca
     5161 attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg
     5221 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc
     5281 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga
     5341 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc
     5401 atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg
     5461 caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag
     5521 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac
     5581 gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct
     5641 ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc
     5701 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt
     5761 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga
     5821 tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat
     5881 tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact
     5941 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc
     6001 tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga
     6061 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg
     6121 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg
     6181 aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc
     6241 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat
     6301 tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag
     6361 tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc
     6421 caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa
     6481 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg
     6541 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg
     6601 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc
     6661 ataaaggcca agaagggcgg aaagatcgcc gtgtaaaaac gttgttgttt ggggttgaat
     6721 tactcttcta gaccgcggtc gagtggctcc ggtgcccgtc agtgggcaga gcgcacatcg
     6781 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
     6841 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     6901 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     6961 gccgccagaa cacaggtgtc gtgacgcgga ccatggcacg gaaggtagat ctcacgagtt
     7021 gtgatagaga accaatacat atcccgggga gcatccaacc atgtggttgc ttgctcgcat
     7081 gcgacgcaca ggctgtaaga ataactcgga tcaccgaaaa tgcaggggcc tttttcggta
     7141 gagaaacccc gagagttggg gagctgctgg ccgattattt tggagagact gaggcacatg
     7201 cattgcgaaa cgcactggcc cagtcaagcg accctaagcg accagctctt atcttcggct
     7261 ggcgcgatgg cttgactggc cgcactttcg atatctccct gcacagacac gatggtacat
     7321 ctatcattga gttcgaaccg gccgcagctg aacaggccga taacccattg agattgacca
     7381 ggcagatcat cgccaggacg aaagaattga agtcactgga ggagatggca gcaagagtcc
     7441 ctaggtattt gcaagcaatg ctgggatacc accgcgttat gctttaccga tttgcagacg
     7501 acgggtccgg tatggtgata ggagaggcca agcgctccga cctggagtca tttcttggcc
     7561 aacactttcc tgccagcctg gtaccccagc aagctaggtt gctctatctc aaaaatgcta
     7621 tacgggtggt gagcgactcc aggggcatta gcagccgcat cgtgcccgag cacgacgcta
     7681 gtggtgccgc cctcgacctt tcctttgctc atctccgaag catctctcca tgccacctgg
     7741 aattcctgag aaacatgggt gtgtcagctt ccatgagcct gtccatcatc atcgacggaa
     7801 ctctctgggg actgattatc tgtcatcatt atgaacctcg cgctgtcccc atggcccaac
     7861 gcgtggcagc tgaaatgttt gccgacttcc tgtctcttca cttcactgcc gctcatcacc
     7921 agaggtgatc tagacccgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     7981 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     8041 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     8101 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     8161 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     8221 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     8281 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     8341 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     8401 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     8461 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     8521 cctccccgca tcgataccgt cgacctcgat cgagacctag aaaaacatgg agcaatcaca
     8581 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     8641 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     8701 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     8761 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg
     8821 cagaactaca caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag
     8881 ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg
     8941 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg
     9001 tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg
     9061 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     9121 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     9181 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc
     9241 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     9301 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     9361 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     9421 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     9481 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     9541 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     9601 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9661 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9721 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9781 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9841 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     9901 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     9961 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    10021 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    10081 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    10141 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    10201 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    10261 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    10321 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    10381 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    10441 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    10501 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    10561 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    10621 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    10681 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    10741 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    10801 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    10861 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    10921 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    10981 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    11041 gtgccacctg ac
//
