LOCUS       sequence-128111-         333 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB204
FEATURES             Location/Qualifiers
     source          1..333
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(83..89,90..107,108..113))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(150..274)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(311..328)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(311..327)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcaac
       61 ggatcctaac tcaaaatcca cacattatac gagccggaag cataaagtgt aaagcctggg
      121 gtgcctaatg cggcccctcg aggggccgcc atagtgactg gatatgttgt gttttacagt
      181 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
      241 acgtttctcg ttcagctttt ttgtacaaac ttgtttgatt agagtcgacc tgcangcatg
      301 caagcttggc actggccgtc gttttacaac gtc
//
