LOCUS       sequence-128137-       10479 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10479)
  AUTHORS   Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B
  TITLE     Streamlined Genome Engineering with a Self-Excising Drug Selection 
            Cassette.
  JOURNAL   Genetics. 2015 Jun 3. pii: genetics.115.178335.
  PUBMED    26044593
REFERENCE   2  (bases 1 to 10479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Jun 3. pii: genetics.115.178335."
COMMENT     Sequence Label: pDD283
FEATURES             Location/Qualifiers
     source          1..10479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    55..76
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(91..96,97..114,115..121)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    129..145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     134..156
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     153..169
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     153..169
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             442..747
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(795..818)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(822..845)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(849..872)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    891..924
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1277..1301
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1302..2327)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(1302..2327)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(2330..2354)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4272..4595
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             complement(6412..6441)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    6451..6484
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(6502..6522)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(7461..7766)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8032..8049)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8032..8048)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8041..8063)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8257..8276)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8376..8398
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8436..8454)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8522..8626
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8627..8695,8696..9487)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8845..8864)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9658..10246
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10147..10166
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10400..10417
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
       61 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgttatcga
      181 tttcctagga acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
      241 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtt
      301 cggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
      361 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgccg
      421 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
      481 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg
      541 atccccctgg ccagtgcacg tctgctgtca gataaagtcc cccgtgaact ttacccggtg
      601 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc
      661 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
      721 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca
      781 ggtcgaacta gtctcttgtc atcgtcatcc ttgtaatcac gcttgtcgtc gtcgtccttg
      841 tagtcacgct tatcgtcatc gtctttataa tcagatccgg ctccctgaaa ataacttcgt
      901 ataatgtatg ctatacgaag ttattaaaaa ttgcggtcat aaactgaaac gtaacatatg
      961 ataaggtatt ttgtgtgcgg ttttttctat gatgtttcaa aaaaatttat cagaagtaaa
     1021 aaacataaga agtgtacaca gaacattgtg tagattttgg gatagaatta ttattaatgg
     1081 ggggagggag cacaattttt ttcgactttt tattacgaat taattctatt tttgaatcta
     1141 cacaatttca ttgttagagg tgacttaaaa gaagctaaaa aacaaagaaa ttaagagaag
     1201 ttttttgata ataacaaaaa tagggggtgg gagcacaggg agaaagagca tgtagggatg
     1261 ttgaagagta attggaccca ctttgtacaa gaaagctggg tctattcctt tgccctcgga
     1321 cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag
     1381 acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg
     1441 attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc
     1501 tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg cgatcctgca
     1561 agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc
     1621 ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag
     1681 tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg
     1741 tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc
     1801 tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga
     1861 tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg
     1921 aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catggcctcc
     1981 gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc
     2041 tgtgcacggc gggagatgca ataggtcagg ctctcgctga attccccaat gtcaagcact
     2101 tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa
     2161 ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa
     2221 gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg
     2281 atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatgga gcctgctttt
     2341 ttgtacaaac ttgcggcacc gcctgacata ttaccttaaa attcaaaaat taatttcaga
     2401 tcagtcataa ccaagaaaac aaaataaacg ttaggacaga aaattcaaat taattaaaaa
     2461 ttatgctgaa taagaaaatt acatgcaaag ccttattttc ctcatagaaa cagttgaata
     2521 acatattttt taattgaaaa catagcaaaa ttcgtggtaa aatttgtcaa aaccttggaa
     2581 aaaatcgaaa aaagaacatt tcccgatgca ttttcctatt tctcgttgcc gacaacgtat
     2641 aaacgttgaa tactcagtca acgaatttgg ctgctggctt ggactctcgc acggaagaga
     2701 cgcgtgtgta gatttacgac gaaagcaaaa atgatcacca aaaacggaac gttgagctgg
     2761 acggaaatag tggtaaagtg acatgattat agtttgaaga tttctaattt cacaattaga
     2821 gcaaatgttg ttcggtattt attttcaacg gtatttatac tattttccac ctttttctag
     2881 aacattcgag ctgcttgttg caaaaggagg gcgactcaca ttcggtacat ggaaaagtag
     2941 tgtacacaat aaagagaccc agatacattt tccgtctgcg tctctttgca cccaccggga
     3001 gtattttcaa acgaatgcat ctaggacctt ctagaacatt ctgtaaggct gcagaatgcg
     3061 ggtatataag gaaagcgggc tcagaggaag ccaacacgct ttgttctagt gcatctaaaa
     3121 aacttcgaaa atatgtcaaa tcttctcact gttcatcaaa atcttccagc acttccagtt
     3181 gacgcgacaa gtgatgaagt tcgcaagaac ctcatggata tgttccgtga cagacaagcc
     3241 ttctccgagc acacctggaa gatgcttctc tcagtgtgcc gttcttgggc tgcctggtgc
     3301 aagcttaaca accgcaagtg gttcccagcc gagccagaag acgttcgtga ctacttgctc
     3361 tacctccaag ctcgtggatt ggcggtgaag acgatccaac aacatctcgg acagctcaat
     3421 atgctccatc gccgatctgg acttccacgc ccatcagatt ctaatgccgt atctctcgta
     3481 atgagacgta tccgcaagga aaatgttgat gctggagaga gagccaagca agccctcgcc
     3541 ttcgaaaggt aagtttaaat attaatgtat tcaatcctaa gacttgacga aattttcaga
     3601 accgacttcg atcaggtccg ttctcttatg gaaaattccg atcgttgcca ggacatccga
     3661 aacctcgcct tcctcggaat tgcttacaac acacttctcc gtatcgccga aattgcccgc
     3721 attcgcgtca aggatattag tcgcacagac ggaggaagaa tgctcatcca catcggacgt
     3781 acaaaaacgc tggtctcgac tgccggagtc gaaaaagcgc tctcccttgg agttaccaag
     3841 ctcgttgagc gctggatttc ggtttccgga gtcgccgatg atccaaacaa ctacctgttc
     3901 tgtcgtgttc gcaagaacgg agttgctgcc ccgagtgcaa catcacaact ctcgactcga
     3961 gctcttgaag gtaagtttaa acaacatatt agctaagaat aagctgaaaa taaattttca
     4021 ggaatcttcg aagctaccca ccgtctcatc tacggagcca aggatgattc cggacaacgt
     4081 tacttggctt ggtctggaca ttccgcccgt gtcggagctg cccgtgacat ggctcgcgct
     4141 ggagtttcca tcccagagat tatgcaagcc ggaggttgga ccaacgtcaa cattgtgatg
     4201 aactatatta gaaacttgga ttctgaaact ggcgccatgg ttcgtcttct cgaagacggt
     4261 gattaagata aatgcaaaat cctttcaagc attcccttct tctctatcac tcttctttct
     4321 ttttgtcaaa aaattctctc gctaatttat ttgctttttt aatgttatta ttttatgact
     4381 ttttatagtc actgaaaagt ttgcatctga gtgaagtgaa tgctatcaaa atgtgattct
     4441 gtctgatgta ctttcacaat ctctcttcaa ttccattttg aagtgcttta aacccgaaag
     4501 gttgagaaaa atgcgagcgc tcaaatattt gtattgtgtt cgttgagtga cccaacaaaa
     4561 agaggaaact ttattgtgcc gccaagaaaa aagtctcact gaatctcaaa tattttatta
     4621 gaaaacacca acaatttaga tatttctgta tccacggttg gcgttgacag cctctgatgg
     4681 tggagacttg tctgggcact ggcagtactc ggcatccttt cctggtcttc cgtctggtcc
     4741 agtctctccc tgggttccgt ctggtccttg acgtccttgc tttccagatg gtccttctgg
     4801 tccaacggtt cctggctctc ctggtggtcc gtgaagaccg ttctgtccag ctggtcctgt
     4861 tggtccttga tctcctcttg gtccctttgg tcctggtctt ccgattggat gctcggcgtc
     4921 gcgtcccttc tctcctggtg gtcctggctc tccgatcttt ccgatttgac cggatgatcc
     4981 ttgctctcct ggatgtcctg gttgtccatc tcttcctggg tttccatttt gtcctcttgg
     5041 tcctggaagt ccacgtggtc ctggtcttcc aagagctcct ggtggtccaa ctggtccttg
     5101 tgggcaagtg aagcatccga cagactcgcg ttgtggagcg atatcatcag ctccaactcc
     5161 tggaacaccg tcaagtcctg gaactccgtc gagtcctgga actcctggaa ctccctttgg
     5221 tccggatggt ccagctgggc acttgttatc agcattgcag ttgcaggttg gtccatttcc
     5281 attggctcca tttggagtct ttggctggtt gaagactggt ggaactgctg gtggttgacc
     5341 tggtggagca gatggggatc catcagcatg tggagttggg gtagcgttgg tctcttcata
     5401 ttgacaacgg acacgcttgc tagatcttcc gatgacaact atgtccttcc acaatccatt
     5461 ggtagattgc tagaaaatta aaaattattt aaaattcaaa atataattta actcactctg
     5521 aacacattca tctcggtatc aagcttctct ctgaatccat caacctccga gtagatggtt
     5581 gacatggtca tgaagcatac cataagagtg gcgacagtga ccgaagccgt cacataacac
     5641 gcaagtttta cagacatctc tgaaattatc aaatttagga tttcaaaata attcggccaa
     5701 agaaatgtaa ctcacctagt cttcgagaag cagagacttg tcgatttcct accggattcg
     5761 tctcctttta ttaggaaatc tttccgcaag ttttcaagtt ttttttgttt tttttttaca
     5821 ttaccgacga gacagtgtgc gagacgcaga gacgataaaa gactggataa gaacgggaga
     5881 taaggagtac ggatgtaggt gaggaatgtg gccgaaagga cagatgggct ttattcggga
     5941 agattggttg ggaaatgaac cagagaagaa tgactgaata aagttgttga aacttggtga
     6001 gaatggaaga tagacaatcg agggcagcta gtttccttcc tcctctatat atttttatgt
     6061 tttcttttaa accttcctcc atatttctga attgaatgtt gttgcatatt gtgtgtcctt
     6121 atttcatttc catcatcctt ttgtgtgttt cccccttcca ccaattcccc ccacgtccta
     6181 atgaaatgaa agcgagaaga taaataaact tgcacattgt gttcagatga gaggagcgga
     6241 atacgaattg ggagacggaa agagaaaaaa aaatgtttag atttggattg aattaatttt
     6301 ttagaaagca agaaaaaaaa cacaaagaga ataaataaat aataggaaaa aaaacaaata
     6361 ttcagtattt aaaatttgaa aattcaacga cgttggcgtc gatcatcctt agaggtcctc
     6421 ctcggagata agcttttgct ctccctgaaa ataacttcgt ataatgtatg ctatacgaag
     6481 ttattttaaa cttacctttc cggattgaaa gtacagattc tccttgtaga gctcgttcat
     6541 tccctcggtg attccggcgg cggtgaggaa ctcaaggagg accatgtggt cacgcttctc
     6601 gtttgggtcc ttgaagagct tggattggta ggagaggtag tggttgtctg ggaggaggac
     6661 tggtccgtct ccgattgggg tgttttgttg gtagtggtcg gcgagttgga ctcctccgtc
     6721 ctcgatgttg tgacggatct gaaaatttaa atcagattag ttagttagta aaatcatgtt
     6781 taaacttacc ttgaagttgg ccttgattcc gttcttttgc ttgtcggcgg tgatgtagac
     6841 gttgtgggag ttgtagttgt actcaagctt gtgtccgagg atgtttccgt cctccttgaa
     6901 gtcgattccc tgaaaattta aatatgtatg cttagttagt accgaactgt ttaaacttac
     6961 cttgagctcg atacggttga cgagggtgtc tccctcgaac ttgacctcgg cacgggtctt
     7021 gtagtttccg tcgtcctgaa aatttaaata atcagggtta gttagtatat atatgtttaa
     7081 acttaccttg aagaagatgg tacgctcttg gacgtatccc tctggcatgg cggacttgaa
     7141 gaagtcgtgt tgcttcatgt ggtctgggta acgggcgaag cattggactc cgtatccgag
     7201 ggtggtgacg agggttggcc atgggactgg gagctttccg gtggtgcaga ggagcttgag
     7261 ggtgagcttt ccgtaggtgg cgtctccctc tccctctccg gagacggaga acttgtgtcc
     7321 gttgacgtct ccgtcgagct cgacgaggat aggtacaact cctgtaaata actcttctcc
     7381 ttttgacatc gatgctcctg aggctcccga tgctccctag gtcgacctgc agactggctg
     7441 tgtataaggg agcctgacat ttatattccc cagaacatca ggttaatggc gtttttgatg
     7501 tcattttcgc ggtggctgag atcagccact tcttccccga taacggagac cggcacactg
     7561 gccatatcgg tggtcatcat gcgccagctt tcatccccga tatgcaccac cgggtaaagt
     7621 tcacggggga ctttatctga cagcagacgt gcactggcca gggggatcac catccgtcgc
     7681 ccgggcgtgt caataatatc actctgtaca tccacaaaca gacgataacg gctctctctt
     7741 ttataggtgt aaaccttaaa ctgcatttca ccagcccctg ttctcgtcgg caaaagagcc
     7801 gttcatttca ataaaccggg cgacctcagc catcccttcc tgattttccg ctttccagcg
     7861 ttcggcacgc agacgacggg cttcattctg catggttgtg cttaccgaac cggagatatt
     7921 gacatcatat atgccttgag caactgatag ctgtcgctgt caactgtcac tgtaatacgc
     7981 tgcttcatag catacctctt tttgacatac ttcgggtaac tagtgccggc gactggccgt
     8041 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     8101 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     8161 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
     8221 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     8281 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     8341 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     8401 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     8461 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     8521 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     8581 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     8641 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     8701 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     8761 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     8821 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     8881 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     8941 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     9001 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     9061 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     9121 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     9181 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     9241 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     9301 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     9361 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     9421 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     9481 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     9541 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     9601 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     9661 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     9721 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     9781 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     9841 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9901 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9961 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
    10021 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
    10081 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
    10141 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
    10201 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    10261 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
    10321 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
    10381 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
    10441 caaaccgcct ctccccgcgc gttggccgat tcattaatg
//
