LOCUS       sequence-128141-         515 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Prakriya M, Feske S, Gwack Y, Srikanth S, Rao A, Hogan PG
  TITLE     Orai1 is an essential pore subunit of the CRAC channel.
  JOURNAL   Nature. 2006 Sep 14. 443(7108):230-3.
  PUBMED    16921383
REFERENCE   2  (bases 1 to 515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2006 Sep 14. 443(7108):230-3."
COMMENT     Sequence Label: human STIM1 CFP
FEATURES             Location/Qualifiers
     source          1..515
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(110..136)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(137..163)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(187..210)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(224..475)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(266..287)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cctcccctac ccggtagaat tcgttaaata ttaaacaggg atttcttgtc tcccacgtgg
       61 gagacctgat actctcaagg gtcccaggtg gggaccctca ctctagttaa gcgtaatccg
      121 gaacatcata tgggtaagcg taatccggaa catcgtatgg gtaagcggcc cgttcgacaa
      181 ttctcacttg tcatcgtcgt ccttgtagtc agcggccgct ttacttgtac agctcgtcca
      241 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
      301 cgttggggtc tttgctcagc ttggactggg tgctcaggta gtggttgtcg ggcagcagca
      361 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
      421 cctcgatgtt gtggcggatc ttgaagttgg ccttgatgcc gttcttctgc ttgtcggcgg
      481 tgatatagac gttgtggctg atgtagttgt actcc
//
