LOCUS       sequence-128143-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Twister plasmid to swap inserts from an alpha1 or alpha1R vector to 
            any omega level vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB1alpha2_SF (GB0107)
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    19..43
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    232..254
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     rep_origin      381..816
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 aacacataca gcgacttagt ttacccgcca atatatcctg tcaagcttcg tctcaagcga
       61 aatgattgat gaagaacaat ggtggatgaa gaacaaagaa ggagggagct tttgttcaag
      121 atgaacaaag aacaatagtg gatgaagaac aaagtgaaaa aaataaaaaa aaatgtatgg
      181 ttaaataaag agtaaagtta ccattgagac tcctgactga gacgaagctt gttacaccac
      241 aatatatcct gccaagatct aattccgggg atcggaaatc cagaagcccg agaggttgcc
      301 gcctttcggg ctttttcttt ttcaaaaaaa aaaatttata aaacgatctg ttgcggccgg
      361 ccgccgggtt gtgggcaaag gcgctcgacg gtgggcaacc gcttgcggtt gtccacgggc
      421 ggagccggtg cgcgtagcgc attgtccaca agccaagggc gaccaataat tgatatatat
      481 attcataatt gaaaagctaa ttgaacatac tacttgctgt aactacttgc cggagcgagg
      541 ggtgtttgca agctgttgat ctgaaagggc tattagcgtt ctcacgtgcc tttttgatta
      601 gcgatttcac gtgaccttat tagcgatttc acgtactccg attagcgatt tcacgtaccc
      661 tgattagcga tttcacgtgg atagtttttg gagcgggccg gaaagccccg tgaatcaagg
      721 ctttgcgggg cattagcggt ttcacgtgga taactaccct ctatccacag gcttccgggg
      781 ataaaaaagc ccgctcgacg gcgggctgtt ggatggggat cgcctgaatc gccc
//
