LOCUS       sequence-128146-         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Twister plasmid to swap inserts from an omega2 or omega2R vector to 
            any alpha level vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB1omega1_SF (GB0158)
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    23..47
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    236..258
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     rep_origin      385..820
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 aacaaacaca tacagcgact tagtttaccc gccaatatat cctgtcagga tccggtctca
       61 tgacagcgaa atgattgatg aagaacaatg gtggatgaag aacaaagaag gagggagctt
      121 ttgttcaaga tgaacaaaga acaatagtgg atgaagaaca aagtgaaaaa aataaaaaaa
      181 aatgtatggt taaataaaga gtaaagttac cattgagctc ctgagaccgg atccgttaca
      241 ccacaatata tcctgccaag atctaattcc ggggatcgga aatccagaag cccgagaggt
      301 tgccgccttt cgggcttttt ctttttcaaa aaaaaaaatt tataaaacga tctgttgcgg
      361 ccggccgccg ggttgtgggc aaaggcgctc gacggtgggc aaccgcttgc ggttgtccac
      421 gggcggagcc ggtgcgcgta gcgcattgtc cacaagccaa gggcgaccaa taattgatat
      481 atatattcat aattgaaaag ctaattgaac atactacttg ctgtaactac ttgccggagc
      541 gaggggtgtt tgcaagctgt tgatctgaaa gggctattag cgttctcacg tgcctttttg
      601 attagcgatt tcacgtgacc ttattagcga tttcacgtac tccgattagc gatttcacgt
      661 accctgatta gcgatttcac gtggatagtt tttggagcgg gccggaaagc cccgtgaatc
      721 aaggctttgc ggggcattag cggtttcacg tggataacta ccctctatcc acaggcttcc
      781 ggggataaaa aagcccgctc gacggcgggc tgttggatgg ggatcgcctg aatcgcccca
      841 tcatccagcc agaaagtgag ggagccacgg ttgatgagag ctttgttgta ggtggaccag
      901 ttggtga
//
