LOCUS       sequence-128148-        3082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Twister plasmid to swap inserts from an omega2 or omega2R vector to 
            any alpha level vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB1omega1_SF (GB0158)
FEATURES             Location/Qualifiers
     source          1..3082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    175..199
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(290..878)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(370..389)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1049..1840)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2484..2919
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    3046..3068
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 ggagctcaat ggtaacttta ctctttattt aaccatacat ttttttttat ttttttcact
       61 ttgttcttca tccactattg ttctttgttc atcttgaaca aaagctccct ccttctttgt
      121 tcttcatcca ccattgttct tcatcaatca tttcgctgtc atgagaccgg atcctgacag
      181 gatatattgg cgggtaaact aagtcgctgt atgtgtttgt ttgagatctc atgtgagcaa
      241 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
      301 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      361 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      421 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      481 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      541 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      601 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      661 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      721 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaagaa
      781 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
      841 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
      901 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
      961 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     1021 gtatatatgt gtaacattgg tctagtgatt atttgccgac taccttggtg atctcgcctt
     1081 tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt
     1141 gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct
     1201 ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc
     1261 aaatgcggga caacgtaagc actacatttc gctcatcacc agcccagtcg ggcggcgagt
     1321 tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa
     1381 agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca
     1441 agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc
     1501 gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt
     1561 cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg
     1621 aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca
     1681 ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt
     1741 acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg
     1801 atagttgagt cgatacttcg gcgatcaccg cttccctcat gatgtttaac tttgttttag
     1861 ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg
     1921 cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa aaacagtcat
     1981 aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg
     2041 accagttgcg tgagcgcata cgctacttgc attacagctt acgaaccgaa caggcttatg
     2101 tccactgggt tcgtgccttc atccgtttcc acggtgtgcg tcacccggca accttgggca
     2161 gcagcgaagt cgaggcattt ctgtcctggc tggaacaccc cttgtattac tgtttatgta
     2221 agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag
     2281 attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt tgaaggatca
     2341 gatcacgcat cttcccgaca acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac
     2401 caactggtcc acctacaaca aagctctcat caaccgtggc tccctcactt tctggctgga
     2461 tgatggggcg attcaggcga tccccatcca acagcccgcc gtcgagcggg cttttttatc
     2521 cccggaagcc tgtggataga gggtagttat ccacgtgaaa ccgctaatgc cccgcaaagc
     2581 cttgattcac ggggctttcc ggcccgctcc aaaaactatc cacgtgaaat cgctaatcag
     2641 ggtacgtgaa atcgctaatc ggagtacgtg aaatcgctaa taaggtcacg tgaaatcgct
     2701 aatcaaaaag gcacgtgaga acgctaatag ccctttcaga tcaacagctt gcaaacaccc
     2761 ctcgctccgg caagtagtta cagcaagtag tatgttcaat tagcttttca attatgaata
     2821 tatatatcaa ttattggtcg cccttggctt gtggacaatg cgctacgcgc accggctccg
     2881 cccgtggaca accgcaagcg gttgcccacc gtcgagcgcc tttgcccaca acccggcggc
     2941 cggccgcaac agatcgtttt ataaattttt ttttttgaaa aagaaaaagc ccgaaaggcg
     3001 gcaacctctc gggcttctgg atttccgatc cccggaatta gatcttggca ggatatattg
     3061 tggtgtaacg gatccggtct ca
//
