LOCUS       sequence-128150-        2753 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Twister plasmid to swap inserts from an alpha1 or alpha1R vector to 
            any omega level vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB1alpha2_SF (GB0107)
FEATURES             Location/Qualifiers
     source          1..2753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    171..195
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(286..874)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(366..385)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1045..1860)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1768..1787
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1852..1871
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      2151..2586
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2713..2735
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 ggagtctcaa tggtaacttt actctttatt taaccataca ttttttttta tttttttcac
       61 tttgttcttc atccactatt gttctttgtt catcttgaac aaaagctccc tccttctttg
      121 ttcttcatcc accattgttc ttcatcaatc atttcgcttg agacgaagct tgacaggata
      181 tattggcggg taaactaagt cgctgtatgt gtttgtttga gatctcatgt gagcaaaagg
      241 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
      301 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      361 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      421 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      481 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      541 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      601 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      661 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
      721 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaagaagagt
      781 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
      841 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
      901 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
      961 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1021 atatgtgtaa cattggtcta gtgattagaa aaactcatcg agcatcaaat gaaactgcaa
     1081 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
     1141 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc
     1201 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
     1261 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc
     1321 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac
     1381 caaaccgtta ttcattcgtg attgcgcctg agcgagtcga aatacgcgat cgctgttaaa
     1441 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac
     1501 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tccctgggat
     1561 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
     1621 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacaa cattggcaac
     1681 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcggta
     1741 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
     1801 atccatgttg gaatttaatc gcggccttga gcaagacgtt tcccgttgaa tatggctcat
     1861 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
     1921 tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt gttgaataaa
     1981 tcgaactttt gctgagttga aggatcagat cacgcatctt cccgacaacg cagaccgttc
     2041 cgtggcaaag caaaagttca aaatcaccaa ctggtccacc tacaacaaag ctctcatcaa
     2101 ccgtggctcc ctcactttct ggctggatga tggggcgatt caggcgatcc ccatccaaca
     2161 gcccgccgtc gagcgggctt ttttatcccc ggaagcctgt ggatagaggg tagttatcca
     2221 cgtgaaaccg ctaatgcccc gcaaagcctt gattcacggg gctttccggc ccgctccaaa
     2281 aactatccac gtgaaatcgc taatcagggt acgtgaaatc gctaatcgga gtacgtgaaa
     2341 tcgctaataa ggtcacgtga aatcgctaat caaaaaggca cgtgagaacg ctaatagccc
     2401 tttcagatca acagcttgca aacacccctc gctccggcaa gtagttacag caagtagtat
     2461 gttcaattag cttttcaatt atgaatatat atatcaatta ttggtcgccc ttggcttgtg
     2521 gacaatgcgc tacgcgcacc ggctccgccc gtggacaacc gcaagcggtt gcccaccgtc
     2581 gagcgccttt gcccacaacc cggcggccgg ccgcaacaga tcgttttata aatttttttt
     2641 tttgaaaaag aaaaagcccg aaaggcggca acctctcggg cttctggatt tccgatcccc
     2701 ggaattagat cttggcagga tatattgtgg tgtaacaagc ttcgtctcag tca
//
