LOCUS       sequence-128160-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses 
            eGFP via P2A cleavage site. EFS Promoter driven.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey 
            ME, Thielke A, Aster JC, Regev A, Ebert BL
  TITLE     Generation of mouse models of myeloid malignancy with combinatorial 
            genetic lesions using CRISPR-Cas9 genome editing.
  JOURNAL   Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951.
  PUBMED    24952903
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951."
COMMENT     Sequence Label: pL-CRISPR.EFS.GFP
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..237
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(283..339)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(355..402)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
       61 gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg
      121 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
      181 gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat
      241 gggcaccacc ccggtgaaca gctcttcgcc cttagacacc atggggccgg ggttctcctc
      301 cacgtcgccg gcctgcttca gcaggctgaa gttggtggcg ccgctgccgc tagctttctt
      361 tttcttagct tgaccagctt tcttagtagc agcaggacgc ttgtcgcctc ccagctgaga
      421 caggtcgatc cgtgtctcgt acaggccggt gatgctctgg tggatcaggg tggcgtccag
      481 cacctctttg gtgctggtgt acctcttccg gtcgatggtg gtgtcaaagt acttgaaggc
      541 ggcaggggct cccagattgg tcagggtaaa caggtggatg atattctcgg cctgctctct
      601 gatgggctta tcccggtgct tgttgtaggc ggacagcact ttgtccagat tagcgtcggc
      661 caggatcact ctcttggaga actcgctgat ctgctcgatg atctcgtcca ggtagtgctt
      721 gtgctgttcc acaaacagct gtttctgctc attatcctcg ggggagccct tcagcttctc
      781 atagtggctg gccaggtaca ggaagttcac atatttggag ggcagggcca gttcgtttcc
      841 cttctgcagt tcgccggcag a
//
